SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. flexibility of monochromator-based systems. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Example: [Drawer]. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Category 2 and 3 microplate readers use gradient heating as well. We would like to show you a description here but the site won’t allow us. Path Check Pathlength Measurement SpectraMax i3x. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The powerful analysis tools in the software generate a standard curve and calculated data. no. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). In addition, the iD5 reader measures TRF and FP. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 0 out of 5 $ 6,499. Summary of Contents for Molecular Devices SpectraMax M2. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. The CellTiter-Glo assay from Promega uses luciferase. Guides: SpectraMax Mini Installation Guide. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The temperature in the plate chamber is ambient and isothermal. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The chamber contains springs that position the cuvette in the proper alignment for a read. Table of Contents. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 4 μL DNA standards. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The 2-channel configuration is available with 0, 2, or 4 injectors. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. 303 unable to cal dark current. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. The instrument uses a paired monochromator and xenon flash. When the user applies a set temperature of 37°C, the top is heated to 37. Next generation SpectraMax® absorbance microplate readers. Most papers they cultured bacteria to OD600>0. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. Molecular Devices Spectramax iD3 Microplate Reader. 2 software: see SoftMax® Pro 7. . Materials. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The dual monochromator optics allow the widest range of. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. ATP standard curves read on SpectraMax microplate readers. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Intended Use/Discipline:Biological. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. The calculated LOQ values were 0. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. Fluorescence Microplate Readers. It features a modular design that allows users to add or change detection cartridges according to their needs. Wide range of applications The VersaMax reader covers. edu) to be added as a trained user in iLab. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. SpectraMax Microplate Readers. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. Fill a clean plate with water. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Open the top cover of the instrument. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Highly customizable low volume. e. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Or scan up to 384 wells at any time to determine the best assay conditions. The SpectraMax® ABS Plus Microplate Reader can run . The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Table of Contents. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. 030 fmol Fluorescence Polarization (FP). SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. SpectraMax i3x Multi-Mode Detection Platform. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 305 reference level saturation. no. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. Step 2: Launch SoftMax Pro Software. Figure 1. Temperature control and shaking come as standard. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. microplate reader. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. This unlocks the transport slide. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. Step 1: Turn on your microplate reader. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Multimode Microplate Readers. 997 (n = 3). Step 4: Incubate at room temperature for 1 hour, protected from light. 40266 Email: drugdiscoverytech@lifetech. In . 1. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. 305 reference level saturation. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Reporter Assay. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. 58% for the detection module and 2. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. 1 software Download page. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Align the two slots on the bottle holder with the two pegs on the rail. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 304 signal level saturation. 003 fmol 0. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. . limit of detection (LLD) for ATP was estimated for each microplate reader using the . A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Figure 1. 020 fmol 0. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. . . The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. . SpectraMax Glo Steady-Luc Reporter Assay Kit. Selection of Water Constant in PathCheck settings. Examples: “Press carefully to engage,” “ Do not press down. The plate reader can measure multiple readouts, including UV and visible absorbance. HTRF-compatible SpectraMax Multi-Mode Readers. The plate. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. SpectraMax iD5 Multi-Mode Microplate Reader. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. No accessories are needed and SoftMax® Pro Software. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. 2 only) SpectraMax M-series Microplate readers. For readers without a cuvette port, these options are not shown in the software. 0 out of 5 $ 5,999. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. The drawing shows a standard microplate of 15 mm height covered with a lid. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. both cuvette-based and microplate reader applications on the same instrument. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular Devices: Molecular Devices. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. begin reading microplates with seven mouse clicks. . Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Microplate Readers. The SpectraMax® ABS Plus Microplate Reader can run . SpectraMax® Microplate Readers and SoftMax® Pro Software. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Microplate reader assays requiring infrared (IR) or red-shifted. flexibility of monochromator-based systems. 2. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Create custom fluorescence assays without having to purchase expensive filter sets. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 0 out of 5 $ 5,999. SpectraMax M5 reader settings are shown. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Endpoint, kinetic, spectrum. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. With optimized reagents, option to operate the. In this product video, see how the SpectraMax M5 quickly reads samples. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. Also see:. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. both cuvette-based and microplate reader applications Microplate Readers. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SoftMax® Pro 7. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Temperature control. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. 1. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Microplate Readers Biotek Epoch Microplate Reader. 303 unable to cal dark current. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Introduction. Download PDF versions of the SpectraMax Microplate Reader User Guides. zip. Thank you!. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. 0 out of 5 $ 9,995 $ 9,495. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. Assays can be better optimized by scanning across. Add to Cart. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The system uses two scanning monochromators to determine the optimal excitation and emission settings. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Cell Analysis. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Lysozyme contains two tryptophan residues that. 0 out of 5 $ 2,999-20%. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. com 4. Italic and boldface type are used for emphasis. The SpectraMax 190 reader can be integrated with Molecular . , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 306 plate air cal fail,low light. These robust readers have been placed in labs from. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax 190 reader can be integrated with Molecular . 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. It was also read on the monochromator-based. If it looks fine, then proceed. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. To resume collecting kinetic measurements within the same file, press the Append button. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. More Product Information. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 7°C (gradient heating design). Published Dec 16, 2020 | Updated May 19, 2023. 100 fmol 1536-well (8 µL) 0. Products. Microplate Automation & Detection. ATP standard curves read on SpectraMax microplate readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). For readers without a cuvette port, these options are not shown in the software. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. Other features and benefits include: Eliminate changing out filters. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax Mini User Guide. For a higher . 380 nm/Exc. 7°C and the bottom to 36. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Compact and tunable absorbance microplate readers DATA SHEET. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The drawing shows a standard microplate of 15 mm height covered with a lid. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The plate reader optics are tunable for absorbance. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The calculated LOQ values were 0. For more sample throughput, both readersSupplier: Molecular Devices. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Microplate readers’ standard detection modes. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. 0 out of 5 $ 5,999. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. Measuring platelet aggregation with a microplate reader. Create custom fluorescence assays without having to purchase expensive filter sets. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. In . The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Published Dec 16, 2020 | Updated Dec 16, 2020. 2. Find our technical support information along with contact details. 1) Select the correct plate and the wells that you want to measure. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. SpectraMax Mini Plug-In for SoftMax Pro 7. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Italic and boldface type are used for emphasis. SAN JOSE, CA — Molecular Devices, LLC. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want.